cfgf23 elisa kit  (Quidel)

Journal: The FASEB Journal

Article Title: Lysophosphatidic Acid Synergizes With 1,25‐Dihydroxyvitamin D to Promote Fibroblast Growth Factor‐23 Synthesis via MAPK Signaling and Induction of the IL12A Gene

doi: 10.1096/fj.202502235R

Figure Lengend Snippet: Stimulation of FGF23 production by LPA and 1,25D and the whole transcriptome analysis of Ocy454 cells. (A) Secreted total FGF23 (cFGF23) concentrations in the culture medium of mouse long bone explants in response to vehicle (VEH), LPA alone, 1,25D alone, or LPA/1,25D after 24 h of stimulation. (B) Serum intact FGF23 (iFGF23) levels following the intraperitoneal LPA injection into the wild‐type and VDR KO littermates. (C) Experiment design for whole transcriptome analysis in Ocy454 cells. (D) Ven diagrams of differentially expressed genes (DEGs) in response to LPA, 1,25D, or LPA/1,25D treatment 2 and 8 h after stimulation. (E) Volcano plots of LPA and 1,25D responsive genes demonstrating LPA and 1,25D act on Ocy454 cells. (F) Expression profile of osteocyte genes in response to LPA, 1,25D, or the LPA/1,25D treatment after 2‐ and 8‐h stimulation. One‐way ANOVA followed by Tukey's (A) or two‐way ANOVA followed by Bonferroni (B) post hoc tests for multiple comparisons. Mean ± SEM, n = 3–4 experiments and n = 7–10 mice/group, respectively. DEGs and adjusted p values obtained by Benjamini‐Hochberg (BH) method using Limma package on R studio (D–F). Blue: 1,25D and Red: LPA responsive genes (E). Asterisks represent * p < 0.05, ** p < 0.01, *** p < 0.001 compared to vehicle‐treated group (F).

Article Snippet: After 24‐h incubation, supernatants were collected, cFGF23 concentrations were measured using a cFGF23 ELISA kit (60‐6300, Quidel) according to the manufacturer's instructions, and the cFGF23 concentrations were normalized by bone weight in the corresponding well.

Techniques: Injection, Expressing

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Phosphatropic hormones after 5 days of low- or high-phosphate (P i ) diet. Levels of A serum parathyroid hormone (PTH), B plasma intact fibroblast growth factor 23 (iFGF23), C plasma C-terminal FGF23 (cFGF23), D the ratio of iFGF23 (ng/l)/cFGF23 (ng/l) as well as serum E calcidiol, and F calcitriol, and G plasma soluble Klotho, and H Fetuin-A on the final day of both the 5-day low and high P i diet. One reference unit (RU)/ml corresponds to 2 ng/l cFGF23. Blue dots represent differences (Δ) between high P i diet and low P i diet expressed as mean difference ± 95% confidence interval and blue dashed line represents the zero line of the right y-axis. Data was analyzed by the paired t -test ( A – E ) or Wilcoxon test ( F–H ), n = 10, * p < 0.05

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Plasma/serum and urine hormones after 5 days of low- or high-phosphate (P i ) diet. Data are presented either as mean ± SD (paired t -test) or median and interquartile range (Wilcoxon test)

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: Pflugers Archiv

Article Title: Controlled dietary phosphate loading in healthy young men elevates plasma phosphate and FGF23 levels

doi: 10.1007/s00424-024-03046-4

Figure Lengend Snippet: Associations between phosphatropic hormones and tubular maximal phosphate reabsorption. Non-linear regression analysis of plasma intact fibroblast growth factor 23 (iFGF23) ( A ), C-terminal FGF23 (cFGF23) ( B ), and serum parathyroid hormone (PTH) ( C ) with tubular maximum phosphate (P i ) reabsorption (TmP/GFR) and of serum calcitriol ( D ), plasma cFGF23 ( E ), and serum PTH ( F ) with plasma iFGF23. A straight black line was fitted and ROUT method for outlier removal was applied. Black and red dots represent participants on low or high P i diet, respectively. Blue dots represent identified outliers. The dashed line represents 95% confidence interval. The slope was analyzed by the extra sum-of-squares F test. Significance level p < 0.05

Article Snippet: Tubular maximum P i reabsorption (TmP/GFR) was calculated by using the fractional tubular reabsorption (TRP) of P i with the following equations using spot urine [ ]: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\begin{array}{l}TRP=1-\left[\left(\frac{{U}_{Pi}}{{P}_{Pi}}\right)*\left(\frac{{P}_{Cr}}{{U}_{Cr}}\right)\right]\\ TRP \le 0.86 \to TmP/GFR=TRP*{P}_{Pi}\\ TRP>0.86 \to TmP/GFR=\left(0.3*\frac{TRP}{1-\left(0.8*TRP\right)}\right)*{P}_{Pi}\end{array}$$\end{document} T R P = 1 - U Pi P Pi ∗ P Cr U Cr T R P ≤ 0.86 → T m P / G F R = T R P ∗ P Pi T R P > 0.86 → T m P / G F R = 0.3 ∗ TRP 1 - 0.8 ∗ T R P ∗ P Pi Daily fractional excretion of P i (FEP i ) was calculated by the following equation using the absolute 24-h urine P i and creatinine: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$FE{P}_{i}=\frac{{(U}_{Pi}*{P}_{Cr})}{{(P}_{Pi}*{U}_{Cr})}*100$$\end{document} F E P i = ( U Pi ∗ P Cr ) ( P Pi ∗ U Cr ) ∗ 100 Daily renal filtered P i load was calculated with the following equation: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Renal\;P_iload/day=\left(\frac{U_{Cr}\ast U_{volume}}{P_{Cr}\ast1440}\right)\ast P_{Pi}$$\end{document} R e n a l P i l o a d / d a y = U Cr * U volume P Cr * 1440 * P Pi Plasma iFGF23, cFGF23, Fetuin-A, and urinary metanephrine were measured with the human iFGF23 and cFGF23 enzyme-linked immunosorbent assay (ELISA) (iFGF23 and cFGF23, Quidel, 60–6600 and 60–6100, respectively), Fetuin-A ELISA (R&D Systems, DFTA00, Lot P413286), and the Metanephrine Urine ELISA (Demeditec Diagnostics GmbH, DEE8400) according to manufacturers’ protocols.

Techniques: Clinical Proteomics

Journal: Nephrology Dialysis Transplantation

Article Title: Spleen contributes significantly to increased circulating levels of fibroblast growth factor 23 in response to lipopolysaccharide-induced inflammation

doi: 10.1093/ndt/gfw376

Figure Lengend Snippet: Effect of acute inflammation on FGF3 regulation: acute inflammation was induced by a single injection (i.p.) of 3.3 μg/kg LPS and mice were sacrificed at hour 3 and 5. Plasma levels of (A) C-terminal FGF23, (B) intact FGF23, (C) TNF-α and (D) NGAL. Data are presented as mean ± SD, n = 4 per group. * P < 0.05 versus control, ** P < 0.01 versus control on ANOVA. Green line = vehicle, red line = LPS.

Article Snippet: FGF23 levels were analyzed in plasma by both a murine iFGF23 ELISA kit that measures the intact active protein exclusively and a cFGF23 ELISA kit (Immutopics, Carlsbad, CA, USA), since these two assays provide different and complementary information.

Techniques: Injection, Clinical Proteomics, Control

Journal: Nephrology Dialysis Transplantation

Article Title: Spleen contributes significantly to increased circulating levels of fibroblast growth factor 23 in response to lipopolysaccharide-induced inflammation

doi: 10.1093/ndt/gfw376

Figure Lengend Snippet: Effect of chronic intermittent, chronic sustained and chronic sustained plus intermittent LPS treatment on FGF23 expression. Chronic intermittent treatment consisted of a 3.3 μg/kg i.p. LPS injection daily for 2 weeks. Chronic sustained treatment consisted of subcutaneous sustained-release LPS pellets delivering 2 mg/kg/day for 2 weeks. Chronic sustained plus intermittent treatment consisted of sustained-release LPS pellets as above plus 3.3 μg/kg i.p. LPS on the day of sacrifice. All mice were sacrificed 5 h after the last injection. Plasma levels of (A) C-terminal FGF23, (B) intact FGF23, (C) TNF-α and (D) NGAL. Data are presented as mean ± SD, n = 5 per group. * P < 0.05 versus control, ** P < 0.01 versus control, *** P < 0.001 versus control on ANOVA. (E) Effect of chronic sustained plus intermittent LPS treatment on FGF23 expression in mice with and without spleen, n = 5 per group. * P = 0.02 versus asplenic mice.

Article Snippet: FGF23 levels were analyzed in plasma by both a murine iFGF23 ELISA kit that measures the intact active protein exclusively and a cFGF23 ELISA kit (Immutopics, Carlsbad, CA, USA), since these two assays provide different and complementary information.

Techniques: Expressing, Injection, Clinical Proteomics, Control